Salam Academy
Past papersCambridge 9700

9700 AS & A Level Biology: past papers

597 papers from 2008 to 2025, with 64 questions sorted by syllabus topic.

Cell structure Paper 2 · 5 questions

Eukaryotic and prokaryotic cell structure4
  1. 9700/22 · May/June 2025Question 114 marks

    Root hair cell structures, and the symplast and apoplast pathways for water crossing the root.

  2. 9700/22 · Oct/Nov 2024Question 217 marks

    Controlling HIV transmission, magnification, and evidence that the opportunistic pathogen Pneumocystis is a eukaryote (cell wall, organelles).

  3. 9700/22 · May/June 2024Question 212 marks

    Organelles of alveolar macrophages, and controlling tuberculosis with BCG vaccination programmes.

  4. 9700/22 · Oct/Nov 2023Question 314 marks

    Liver blood supply, hepatocyte organelles, Kupffer cells removing old red cells, and peroxisomes vs mitochondria.

Revise the lesson: Eukaryotic and prokaryotic cell structure
Microscopy and magnification2
  1. 9700/22 · Oct/Nov 2024Question 217 marks

    Controlling HIV transmission, magnification, and evidence that the opportunistic pathogen Pneumocystis is a eukaryote (cell wall, organelles).

  2. 9700/22 · May/June 2024Question 114 marks

    Smooth muscle in airways and the gut wall, cell size and magnification, and what a gene mutation is.

Revise the lesson: Microscopy and magnification

Biological molecules Paper 2 · 5 questions

Carbohydrates and lipids2
  1. 9700/22 · May/June 2025Question 310 marks

    Galactose compared with α-glucose, and analysing sugar content data for carrot varieties.

  2. 9700/22 · Oct/Nov 2024Question 18 marks

    Fatty acids in olive oil: saturated and unsaturated chains, and the structure and variety of triglycerides.

Revise the lesson: Carbohydrates and lipids
Proteins and water3
  1. 9700/22 · May/June 2025Question 27 marks

    Haemoglobin as a globular protein, and the substances involved in carrying oxygen and carbon dioxide in blood.

  2. 9700/22 · May/June 2024Question 417 marks

    Using photosynthesis products, nitrate uptake by roots, and loading amino acids and sucrose into phloem.

  3. 9700/22 · Oct/Nov 2023Question 212 marks

    Keratin structure with disulfide bonds, and comparing keratinase enzymes for temperature and pH range.

Revise the lesson: Proteins and water

Enzymes Paper 2 · 3 questions

Enzyme action and kinetics3
  1. 9700/22 · May/June 2025Question 610 marks

    Non-competitive inhibition and Km for horseradish peroxidase, and start and stop codons in a synthetic gene.

  2. 9700/22 · May/June 2024Question 36 marks

    Lysozyme and the induced-fit model, and comparing lysozyme with penicillin as antibacterial agents.

  3. 9700/22 · Oct/Nov 2023Question 212 marks

    Keratin structure with disulfide bonds, and comparing keratinase enzymes for temperature and pH range.

Revise the lesson: Enzyme action and kinetics

Cell membranes and transport Paper 2 · 3 questions

Transport across membranes3
  1. 9700/22 · Oct/Nov 2024Question 67 marks

    The apoplast pathway and the Casparian strip, and active transport vs facilitated diffusion of potassium ions into root cells.

  2. 9700/22 · May/June 2024Question 417 marks

    Using photosynthesis products, nitrate uptake by roots, and loading amino acids and sucrose into phloem.

  3. 9700/22 · Oct/Nov 2023Question 412 marks

    Maintaining diffusion gradients in alveoli, ventilation, surfactant, and transcription and translation of a transporter gene.

Revise the lesson: Transport across membranes

The mitotic cell cycle Paper 2 · 4 questions

Mitosis and the cell cycle4
  1. 9700/22 · May/June 2025Question 57 marks

    Using antibodies on magnetic beads to collect stem cells, and making artificial red blood cells.

  2. 9700/22 · Oct/Nov 2024Question 413 marks

    Adult stem cells, tissue repair by mitosis, tumour formation, telomeres, and blood cells formed from bone marrow stem cells.

  3. 9700/22 · May/June 2024Question 55 marks

    Chromatin condensing into chromosomes during mitosis.

  4. 9700/22 · Oct/Nov 2023Question 17 marks

    Chromatin and histones, chromosome structure at metaphase, and changes to chromosomes from S phase through mitosis.

Revise the lesson: Mitosis and the cell cycle

Nucleic acids and protein synthesis Paper 2 · 4 questions

DNA structure and replication1
  1. 9700/22 · Oct/Nov 2024Question 35 marks

    Complementary base pairing between DNA and RNA nucleotides during transcription.

Revise the lesson: DNA structure and replication
Transcription and translation4
  1. 9700/22 · May/June 2025Question 610 marks

    Non-competitive inhibition and Km for horseradish peroxidase, and start and stop codons in a synthetic gene.

  2. 9700/22 · Oct/Nov 2024Question 35 marks

    Complementary base pairing between DNA and RNA nucleotides during transcription.

  3. 9700/22 · May/June 2024Question 114 marks

    Smooth muscle in airways and the gut wall, cell size and magnification, and what a gene mutation is.

  4. 9700/22 · Oct/Nov 2023Question 412 marks

    Maintaining diffusion gradients in alveoli, ventilation, surfactant, and transcription and translation of a transporter gene.

Revise the lesson: Transcription and translation

Transport in plants Paper 2 · 4 questions

Xylem, phloem and transpiration4
  1. 9700/22 · May/June 2025Question 114 marks

    Root hair cell structures, and the symplast and apoplast pathways for water crossing the root.

  2. 9700/22 · Oct/Nov 2024Question 67 marks

    The apoplast pathway and the Casparian strip, and active transport vs facilitated diffusion of potassium ions into root cells.

  3. 9700/22 · May/June 2024Question 417 marks

    Using photosynthesis products, nitrate uptake by roots, and loading amino acids and sucrose into phloem.

  4. 9700/22 · Oct/Nov 2023Question 66 marks

    Xylem vessel elements, cohesion-tension, and how xylem structure reduces resistance to flow.

Revise the lesson: Xylem, phloem and transpiration

Transport in mammals Paper 2 · 5 questions

Blood vessels and haemoglobin4
  1. 9700/22 · May/June 2025Question 27 marks

    Haemoglobin as a globular protein, and the substances involved in carrying oxygen and carbon dioxide in blood.

  2. 9700/22 · Oct/Nov 2024Question 510 marks

    Malaria and the oxygen dissociation curve: standardising an experiment and the effect of a right shift on loading and unloading.

  3. 9700/22 · Oct/Nov 2023Question 314 marks

    Liver blood supply, hepatocyte organelles, Kupffer cells removing old red cells, and peroxisomes vs mitochondria.

  4. 9700/22 · Oct/Nov 2023Question 59 marks

    Tuberculosis species and transmission between people and animals, and spread to arteries.

Revise the lesson: Blood vessels and haemoglobin
The heart and cardiac cycle1
  1. 9700/22 · May/June 2024Question 66 marks

    HIV/AIDS and antiretroviral therapy, and a coronary artery bypass graft.

Revise the lesson: The heart and cardiac cycle

Gas exchange Paper 2 · 3 questions

Gas exchange surfaces3
  1. 9700/22 · May/June 2025Question 412 marks

    Tuberculosis: the pathogen, transmission, antibiotic treatment, and identifying airways and blood vessels in lung tissue.

  2. 9700/22 · May/June 2024Question 114 marks

    Smooth muscle in airways and the gut wall, cell size and magnification, and what a gene mutation is.

  3. 9700/22 · Oct/Nov 2023Question 412 marks

    Maintaining diffusion gradients in alveoli, ventilation, surfactant, and transcription and translation of a transporter gene.

Revise the lesson: Gas exchange surfaces

Infectious diseases Paper 2 · 7 questions

Infectious diseases and antibiotics7
  1. 9700/22 · May/June 2025Question 412 marks

    Tuberculosis: the pathogen, transmission, antibiotic treatment, and identifying airways and blood vessels in lung tissue.

  2. 9700/22 · Oct/Nov 2024Question 217 marks

    Controlling HIV transmission, magnification, and evidence that the opportunistic pathogen Pneumocystis is a eukaryote (cell wall, organelles).

  3. 9700/22 · Oct/Nov 2024Question 510 marks

    Malaria and the oxygen dissociation curve: standardising an experiment and the effect of a right shift on loading and unloading.

  4. 9700/22 · May/June 2024Question 212 marks

    Organelles of alveolar macrophages, and controlling tuberculosis with BCG vaccination programmes.

  5. 9700/22 · May/June 2024Question 36 marks

    Lysozyme and the induced-fit model, and comparing lysozyme with penicillin as antibacterial agents.

  6. 9700/22 · May/June 2024Question 66 marks

    HIV/AIDS and antiretroviral therapy, and a coronary artery bypass graft.

  7. 9700/22 · Oct/Nov 2023Question 59 marks

    Tuberculosis species and transmission between people and animals, and spread to arteries.

Revise the lesson: Infectious diseases and antibiotics

Immunity Paper 2 · 3 questions

Immune response and vaccination3
  1. 9700/22 · May/June 2025Question 57 marks

    Using antibodies on magnetic beads to collect stem cells, and making artificial red blood cells.

  2. 9700/22 · Oct/Nov 2024Question 413 marks

    Adult stem cells, tissue repair by mitosis, tumour formation, telomeres, and blood cells formed from bone marrow stem cells.

  3. 9700/22 · May/June 2024Question 212 marks

    Organelles of alveolar macrophages, and controlling tuberculosis with BCG vaccination programmes.

Revise the lesson: Immune response and vaccination

Energy and respiration Paper 4 · 5 questions

ATP, glycolysis and anaerobic respiration2
  1. 9700/42 · May/June 2025Question 107 marks

    Blood lactate in a diving grey seal and anaerobic respiration.

  2. 9700/42 · Oct/Nov 2023Question 19 marks

    Ethanol and lactate fermentation, why they regenerate NAD, and adaptations of rice to flooded fields.

Revise the lesson: ATP, glycolysis and anaerobic respiration
Krebs cycle and oxidative phosphorylation3
  1. 9700/42 · May/June 2025Question 110 marks

    The electron transport chain in the inner mitochondrial membrane, chemiosmosis and ATP synthesis.

  2. 9700/42 · Oct/Nov 2024Question 67 marks

    Using DCPIP as a redox indicator to measure the effect of temperature on yeast respiration.

  3. 9700/42 · May/June 2024Question 611 marks

    Oxygen entering the mitochondrion, the electron transport chain and ATP synthase, and mitochondrial DNA.

Revise the lesson: Krebs cycle and oxidative phosphorylation

Photosynthesis Paper 4 · 3 questions

Light-dependent reactions3
  1. 9700/42 · Oct/Nov 2024Question 713 marks

    Chromatography of chloroplast pigments, the role of accessory pigments, and the light-independent stage.

  2. 9700/42 · May/June 2024Question 79 marks

    Temperature and light intensity as limiting factors, and photolysis in non-cyclic photophosphorylation.

  3. 9700/42 · Oct/Nov 2023Question 812 marks

    Non-cyclic photophosphorylation, uses of Calvin cycle intermediates, and temperature as a limiting factor.

Revise the lesson: Light-dependent reactions
Calvin cycle and limiting factors3
  1. 9700/42 · Oct/Nov 2024Question 713 marks

    Chromatography of chloroplast pigments, the role of accessory pigments, and the light-independent stage.

  2. 9700/42 · May/June 2024Question 79 marks

    Temperature and light intensity as limiting factors, and photolysis in non-cyclic photophosphorylation.

  3. 9700/42 · Oct/Nov 2023Question 812 marks

    Non-cyclic photophosphorylation, uses of Calvin cycle intermediates, and temperature as a limiting factor.

Revise the lesson: Calvin cycle and limiting factors

Homeostasis Paper 4 · 7 questions

Homeostasis and the kidney4
  1. 9700/42 · Oct/Nov 2024Question 19 marks

    Ultrafiltration in the glomerulus and selective reabsorption in the proximal convoluted tubule.

  2. 9700/42 · Oct/Nov 2024Question 1010 marks

    Principles of homeostasis for blood glucose, and how a glycogen phosphorylase mutation affects glucagon signalling.

  3. 9700/42 · May/June 2024Question 18 marks

    Osmoregulation: osmoreceptors, ADH and its effect on urine production.

  4. 9700/42 · Oct/Nov 2023Question 98 marks

    ADH concentration after drinking water, and how the kidney responds.

Revise the lesson: Homeostasis and the kidney
Blood glucose control4
  1. 9700/42 · May/June 2025Question 512 marks

    Obtaining human genes for recombinant proteins, and producing insulin to treat diabetes.

  2. 9700/42 · Oct/Nov 2024Question 1010 marks

    Principles of homeostasis for blood glucose, and how a glycogen phosphorylase mutation affects glucagon signalling.

  3. 9700/42 · May/June 2024Question 108 marks

    Negative feedback in blood glucose control, glucagon action, and urine test strips for glucose.

  4. 9700/42 · Oct/Nov 2023Question 614 marks

    Glucagon and the enzyme cascade in liver cells, and testing glucose with urine strips and biosensors.

Revise the lesson: Blood glucose control

Control and coordination Paper 4 · 5 questions

Nerve impulses and synapses4
  1. 9700/42 · May/June 2025Question 711 marks

    Maintaining a resting potential, and how low blood potassium changes an action potential.

  2. 9700/42 · Oct/Nov 2024Question 811 marks

    Structure of a motor neurone, the neuromuscular junction and the order of events in muscle contraction.

  3. 9700/42 · May/June 2024Question 815 marks

    Neurones and myelin, impulse transmission, and how morphine acts at a cholinergic synapse.

  4. 9700/42 · Oct/Nov 2023Question 79 marks

    Mitochondria at neuromuscular junctions and in muscle, excitatory and inhibitory synapses, and multiple sclerosis.

Revise the lesson: Nerve impulses and synapses
Muscle contraction and plant responses3
  1. 9700/42 · May/June 2025Question 612 marks

    Guard cell structure, daily rhythms of stomatal opening, and how stomata open and close.

  2. 9700/42 · Oct/Nov 2024Question 811 marks

    Structure of a motor neurone, the neuromuscular junction and the order of events in muscle contraction.

  3. 9700/42 · Oct/Nov 2023Question 79 marks

    Mitochondria at neuromuscular junctions and in muscle, excitatory and inhibitory synapses, and multiple sclerosis.

Revise the lesson: Muscle contraction and plant responses

Inheritance Paper 4 · 9 questions

Meiosis and variation4
  1. 9700/42 · May/June 2025Question 48 marks

    Meiosis, fertilisation and why a zygote is diploid with homologous chromosomes.

  2. 9700/42 · Oct/Nov 2024Question 512 marks

    Continuous and discontinuous variation, causes of variation, and natural selection.

  3. 9700/42 · May/June 2024Question 29 marks

    Causes of phenotypic variation and how variation allows evolution.

  4. 9700/42 · Oct/Nov 2023Question 214 marks

    The yeast life cycle: budding by mitosis, meiosis and spores, and why diploid cells hide harmful recessive alleles.

Revise the lesson: Meiosis and variation
Monohybrid and dihybrid inheritance5
  1. 9700/42 · May/June 2025Question 314 marks

    Awns on rice grains: a dihybrid cross with epistasis and a chi-squared test.

  2. 9700/42 · Oct/Nov 2024Question 46 marks

    A monohybrid genetic diagram for tomato fruit colour and the ratios from a dihybrid cross.

  3. 9700/42 · May/June 2024Question 311 marks

    Colour patterns in tortoise beetles: genetic diagrams, codominance and a chi-squared test.

  4. 9700/42 · May/June 2024Question 411 marks

    Gene, protein and phenotype in haemophilia, and gibberellin and stem height in peas.

  5. 9700/42 · Oct/Nov 2023Question 59 marks

    A dihybrid cross in fruit flies with linked genes and a chi-squared test.

Revise the lesson: Monohybrid and dihybrid inheritance

Selection and evolution Paper 4 · 5 questions

Natural selection and population genetics5
  1. 9700/42 · May/June 2025Question 26 marks

    How genetic drift and the founder effect change allele frequencies.

  2. 9700/42 · Oct/Nov 2024Question 29 marks

    Processes that change allele frequencies, and selective breeding of maize for vigour and uniform height.

  3. 9700/42 · Oct/Nov 2024Question 512 marks

    Continuous and discontinuous variation, causes of variation, and natural selection.

  4. 9700/42 · May/June 2024Question 29 marks

    Causes of phenotypic variation and how variation allows evolution.

  5. 9700/42 · Oct/Nov 2023Question 37 marks

    Selective breeding of wheat for disease resistance.

Revise the lesson: Natural selection and population genetics

Classification, biodiversity and conservation Paper 4 · 6 questions

Classification and biodiversity4
  1. 9700/42 · May/June 2025Question 811 marks

    Features of the kingdom Fungi, and how the partners in a lichen benefit each other.

  2. 9700/42 · May/June 2025Question 99 marks

    Sampling plants with a frame quadrat, and testing for a correlation between plant height and altitude.

  3. 9700/42 · Oct/Nov 2023Question 214 marks

    The yeast life cycle: budding by mitosis, meiosis and spores, and why diploid cells hide harmful recessive alleles.

  4. 9700/42 · Oct/Nov 2023Question 108 marks

    Mark-release-recapture to estimate population size, and Himalayan balsam as an invasive species.

Revise the lesson: Classification and biodiversity
Conservation and human impact3
  1. 9700/42 · Oct/Nov 2024Question 910 marks

    Why the red ruffed lemur is critically endangered, and captive breeding programmes in zoos.

  2. 9700/42 · May/June 2024Question 98 marks

    An invasive toad in Madagascar, and IVF in conserving the eastern black rhino.

  3. 9700/42 · Oct/Nov 2023Question 108 marks

    Mark-release-recapture to estimate population size, and Himalayan balsam as an invasive species.

Revise the lesson: Conservation and human impact

Genetic technology Paper 4 · 4 questions

Recombinant DNA and PCR3
  1. 9700/42 · May/June 2025Question 512 marks

    Obtaining human genes for recombinant proteins, and producing insulin to treat diabetes.

  2. 9700/42 · Oct/Nov 2024Question 313 marks

    Genetic engineering and gene therapy for an inherited eye disease, and why the eye suits gene therapy.

  3. 9700/42 · Oct/Nov 2023Question 410 marks

    Fluorescent marker genes in genetic engineering, and DNA microarrays for gene expression.

Revise the lesson: Recombinant DNA and PCR
Genetic engineering applications4
  1. 9700/42 · May/June 2025Question 512 marks

    Obtaining human genes for recombinant proteins, and producing insulin to treat diabetes.

  2. 9700/42 · Oct/Nov 2024Question 313 marks

    Genetic engineering and gene therapy for an inherited eye disease, and why the eye suits gene therapy.

  3. 9700/42 · May/June 2024Question 510 marks

    BRCA1 mutations, genetic screening and cancer risk, and survival data after drug treatment.

  4. 9700/42 · Oct/Nov 2023Question 410 marks

    Fluorescent marker genes in genetic engineering, and DNA microarrays for gene expression.

Revise the lesson: Genetic engineering applications